The new trailer shows off a variety of locations from the sunny coastal town, including a medieval church, sprawling mansion and busy urban areas.
"Sapienza is a dense location full of challenges and packed with opportunities and stories for the enterprising player to unfold. And as with all locations within Hitman, dark secrets and engaging stories are everywhere to be found in charming Sapienza."
There will no doubt be plenty of opportunity for creative murder as you hunt down and assassinate your targets.
Related: Yokai Watch Review
The Sapienza location was originally intended to be playable at launch, until IO Interactive decided to make Hitman fully episodic.
Hitman’s third episode, set in Morocco, is coming in May. If you can’t wait that long, a number of free update are due in the coming weeks alongside the second episode.
Square Enix plans to release every episode before the end of 2016, with a physical release and epilogue to come next year.